Proteins are biological macromolecules
considered as the basic component of a majority of enzymes and other
complex bimolecular compounds, and are also required in the synthesis of
such compounds. Proteins differ from one another based on the number of
peptide structures present within them and they way in which they are
folded ore arranged. These peptides are further composed of amino acid
molecules that together form a chain to give rise to the peptide’s
respective structure. Protein electrophoresis has been a fundamental
research process to evaluate the contents of a protein based on their
molecular weight and their charge. Protein electrophoresis can either be
done by either denaturing or non denaturing methods. Denaturing process
allows protein unfolding and study of various polypeptides by their
molecular weight and charge (charge to mass ratio), whereas non
denaturing methods (also known as native gel methods) take protein size
into consideration as a whole without unfolding the main structure
thereby studying its shape and size in the process.
Polyacrylamide gel is a commonly used
porous gelling agent, for both methods, that allows for movement of
proteins within the buffer medium. Denaturing methods employ sodium
dodecyl suphate (SDS), a commonly used detergent agent that allows for
unfolding of proteins into their polypeptides and in the process assists
free movement of ionized protein molecules in the water medium.
Together SDS polyacryilamide gel electrophoresis (SDS-PAGE) is the most
common denaturing gel method for protein electrophoresis. Among native
PAGE methods blue native and clear native PAGE methods are most common
and inexpensive procedures, they are followed by the highly accurate
quantitative preparative native continuous PAGE (QPNC-PAGE) that employs
Nuclear Magnetic Resonance spectrometer to study molecules of protein
polypeptide. Blue native (BN-PAGE) process requires the assistance of a
Coomassie brilliant blue dye that provides charges to proteins with weak
charge and also makes it easier to spot, however they have been known
to denature proteins in the process. Clear native (CN-PAGE) process is
therefore used in cases where such denaturing is to be avoided
completely. Although they have lower visibility of the proteins, CN-PAGE
is much more reliable than BN PAGE offering micro scale separation and
retaining supramolecular structure assembly of polypeptide chains.
Globally, the Asia Pacific region is
currently the largest market for the basic protein electrophoresis
equipment by sales volume. This is followed by North America, Europe and
consecutively the rest of the world (RoW) region. The Asian markets
home to a large number of biotechnological analytic device manufacturing
companies in the world. The increased spending by the governments of
the emerging countries in Asia in research and technology assists the
rise in demand for protein electrophoresis equipment in the region which
was earlier met by Japanese and European Companies. Other Asian players
are expected to enter the market in the near future thereby reducing
the cost and increasing the market for the years to come. Raw materials
and reagents required for the same are provided by a majority of
European and Asian manufacturers.
The market players in this industry are
numerous as the markets are highly fragmented depending upon their
region of operation. Some noted players in the global market include;
CBS Scientific Company Inc., Cleaver Scientific ltd., Serva
Electrophoresis GmbH., LabRepCo., Sigma-Aldrich Co. LLC., Lonza Group
Ltd, Hoefer Inc., etc, to name a few.
This report is a complete study of
current trends in the market, industry growth drivers, and restraints.
It provides market projections for the coming years. It includes
analysis of recent developments in technology, Porter’s five force model
analysis and detailed profiles of top industry players. The report also
includes a review of micro and macro factors essential for the existing
market players and new entrants along with detailed value chain
analysis.
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